Data underlying the manuscript: Self-mixing in microtubule-kinesin active fluid from nonuniform to uniform distribution of activity

This database presents the data used in the manuscript: Self-mixing in microtubule-kinesin active fluid from nonuniform to uniform distribution of activity (Bate et al. <em>Nat Commun</em> <strong>13</strong>, 6573 [2022]). This database contains two sets of data: (1) images of tracer particles suspended in active-inactive fluid systems and (2) images of fluorescein suspended in active-inactive fluid systems. Each data set is provided with associated analysis script for demonstrating the process of extracting mixing-related parameters from the data, such as interface progression exponents, interface progression coefficients and mixing times. <strong>The first data set</strong> contains 4 files: Data1.zip, analysis1.m, Mask.tif, and Tracking.mat. Data1.zip contains 1 hour of frame-by-frame tracer data from an individual experiment in the [cATP] sweep in Fig. 2c of the manuscript, for the sample with 5 mM caged ATP. The tracers are tracked using the tracking software developed by Ouellette et al. (Experiments in Fluids <strong>40</strong>, 301-313 [2005]) and the tracking results are output in Tracking.mat, which can be subsequently imported to MATLAB by running analysis1.m (requiring MATLAB Curve Fitting Toolbox, Image Processing Toolbox and Parallel Computing Toolbox). The script outputs the interface progression exponents and interface progression coefficient of this data set. The script also allows for analyzing a specific region of interest defined as the black pixels in Mask.tif. In this analysis, the region of interest is set to be the entire image. <strong>The second data set</strong> contains 2 files: Data2.zip and analysis2.m. Data2.zip contains 1 hour of frame-by-frame fluorescein data from an individual experiment in the fluorescein sweep in Fig. 5 of the manuscript. The sample contains 3.2 mM caged ATP, 3 µM caged fluorescein, 80 nM K365 dimers and the sample height is 100 µm. This experiment is designed to probe the mixing time of suspended fluorescein in the active fluid system. To extract the mixing time, Data2.zip needs to be unzipped and analysis2.m needs to be run in MATLAB (requiring MATLAB Curve Fitting Toolbox and Image Processing Toolbox), which will read the fluorescein images to analyze the multiscale norm and output the mixing time.

本数据集配套于论文《活性分布从非均匀到均匀的微管-驱动蛋白活性流体中的自混合效应》（Bate 等，*Nature Communications（Nat Commun）* **13**, 6573 [2022]）中所使用的实验数据。本数据集包含两类数据：(1) 悬浮于活性-非活性流体体系中的示踪粒子图像；(2) 悬浮于活性-非活性流体体系中的荧光素图像。每一类数据均配套有相应的分析脚本，用于演示从数据中提取与混合相关参数的流程，包括界面演化指数、界面演化系数与混合时间。**第一类数据集**包含4个文件：Data1.zip、analysis1.m、Mask.tif 与 Tracking.mat。Data1.zip 包含本论文图2c中[笼状ATP（caged ATP）]浓度扫描实验中单个样本（5 mM 笼状ATP）的1小时逐帧示踪数据。示踪粒子的跟踪采用Ouellette 等人开发的跟踪软件（*Experiments in Fluids* **40**, 301-313 [2005]），跟踪结果输出至Tracking.mat，可通过运行analysis1.m（需依赖MATLAB曲线拟合工具箱（Curve Fitting Toolbox）、图像处理工具箱（Image Processing Toolbox）与并行计算工具箱（Parallel Computing Toolbox））将其导入MATLAB进行分析。该脚本可输出本数据集的界面演化指数与界面演化系数，同时支持对Mask.tif中定义为黑色像素的特定感兴趣区域进行分析，本次分析中将感兴趣区域设置为整张图像。**第二类数据集**包含2个文件：Data2.zip 与 analysis2.m。Data2.zip 包含本论文图5中荧光素扫描实验中单个实验的1小时逐帧荧光素数据。该样本包含3.2 mM 笼状ATP、3 µM 笼状荧光素、80 nM K365二聚体，样本高度为100 µm。本实验旨在探究活性流体体系中悬浮荧光素的混合时间。如需提取混合时间，需先解压Data2.zip，再在MATLAB中运行analysis2.m（需依赖MATLAB曲线拟合工具箱与图像处理工具箱），该脚本将读取荧光素图像以分析多尺度范数，并输出混合时间。