Expression data from FAK null mouse embryonic fibroblasts treated with endothelin-1

Endothelin-1 (ET-1) plays a critical role in connective tissue remodeling by fibroblasts during tissue repair and fibrosis. We investigated the molecular pathways in the transmission of ET-1 signals that lead to features of connective tissue remodeling, in particular the role of FAK (focal adhesion kinase). We used microarrays to investigated whether FAK is required for ET-1 to promote the global programme of gene expression underlying myofibroblast formation and identified distinct classes of up-regulated genes during this process. Genes whose induction by ET-1 required FAK Affymetrix Mouse Genome 430 2.0 GeneChips were used to identify differential gene expression changes bewteen samples.

内皮素-1（Endothelin-1, ET-1）在组织修复与纤维化进程中，可通过成纤维细胞介导结缔组织重塑，发挥关键调控作用。本研究旨在解析ET-1信号传导引发结缔组织重塑特征的分子通路，尤其聚焦黏着斑激酶（focal adhesion kinase, FAK）的功能角色。我们借助基因微阵列技术，探究FAK是否为ET-1驱动肌成纤维细胞形成相关全局基因表达程序所必需，并鉴定了该过程中上调表达的不同基因类别。针对ET-1诱导表达依赖FAK的基因，本研究采用Affymetrix小鼠基因组430 2.0基因芯片，以识别不同样本间的差异基因表达变化。