Intrinsic differences between oral and skin keratinocytes. Mus musculus

The morphology and the behavior of skin and oral tissue keratinocytes are different. One significant dissimilarity between the two sites is the response to injury. Oral and skin keratinocytes have intrinsic differences in the response to injury and such differences are reflected in gene expression profiles. We used microarrays to investigate differences in global gene expression patterns between baseline skin and oral epithelium sheets without their underlying connective tissue. Overall design: Paired skin and oral epithelium was separated from the dermis for RNA extraction and hybridization on Affymetrix microarrays. Skin epidermal tissues were obtained from the tail of mice and oral epidermal tissues were obtained from the hard palate. Enzymatically isolated epithelium was used for analysis.

皮肤与口腔组织角质形成细胞（keratinocytes）的形态学特征及生物学行为存在显著差异。二者之间一项关键差异体现在损伤应答反应上。口腔与皮肤角质形成细胞在损伤应答方面存在固有差异，这种差异可通过基因表达谱得以体现。本研究采用微阵列芯片（microarrays）技术，探究了剥离下层结缔组织的基线状态皮肤及口腔上皮薄片的全局基因表达模式差异。
实验整体设计：将配对的皮肤与口腔上皮组织从真皮层分离，提取RNA后在Affymetrix微阵列芯片上进行杂交实验。皮肤表皮组织取自小鼠尾部，口腔表皮组织取自小鼠硬腭。经酶解法分离得到的上皮组织用于后续分析。