Dissociation constants (Kd) of the interaction between DLG1 PDZ domains and APC-C11/APC-C4 peptides.

ITC experiments were performed at 25°C. Typically, a 400 µM PDZ domain of DLG1 was injected into a 40 µM GST-tagged APC-C11 or APC-C4 protein solution. “n/a” refers to that no detectable interaction was observed. A negative value for ΔH indicates an exothermic reaction while a positive value for ΔH indicates an endothermic reaction.

等温滴定量热法（Isothermal Titration Calorimetry，ITC）实验均于25℃条件下开展。常规实验方案为：将浓度为400 μM的DLG1 PDZ结构域（PDZ domain）注射至40 μM的谷胱甘肽S-转移酶（Glutathione S-Transferase，GST）标记的APC-C11或APC-C4蛋白溶液中。标注"n/a"即表示未检测到可观测的分子相互作用。焓变（ΔH）为负值时代表反应为放热反应，正值则代表吸热反应。