NEON Biorepository Benthic Macroinvertebrate Collection (Unsorted Bulk Sample)

This collection contains unsorted bulk benthic macroinvertebrate samples collected in the field (NEON sample class: inv_fielddata_in.geneticSampleID). Benthic macroinvertebrate samples are collected three times per year at wadeable stream, river, and lake sites during aquatic biology bout windows, roughly in spring, summer, and fall. Samples are collected using the most appropriate sampler for the habitat type, including Surber, Hess, hand corer, modified kicknet, D-frame sweep, and petite ponar samplers. Macroinvertebrates are sampled using a percent-based macrohabitat approach. In wadeable streams, samples are collected in the two most dominant habitat types (e.g. riffles, runs, pools, step pools) within each 1 km-long wadeable stream site. In lakes, samples are collected near the buoy, inlet, and outlet sensors using a petite ponar, and in littoral areas using a D-frame sweep. In rivers, samples are collected near the buoy and two other deep-water locations using a petite ponar sampler, and in littoral areas using a D-frame sweep or large-woody debris sampler. Field protocols differ depending on the habitat and substrate being sampled, but all samples are collected from the surface of the natural substratum in each habitat. Samples are preserved in ethanol in the field, and returned to the domain support facility for a preservative change prior to shipping. The DNA samples from bout 2 (summer) are sent to an external laboratory for metabarcoding. The unsorted bulk DNA samples from bout 1 (spring) and bout 3 (autumn) are archived for future research. Unsorted bulk macroinvertebrate samples are preserved in 70-95% ethanol and archived in a temperature (17-18°C) and humidity controlled environment. See related links below for protocols and NEON related data products.

本数据集收录野外采集的未分拣大型底栖无脊椎动物（benthic macroinvertebrate）批量样本（NEON样本类别：inv_fielddata_in.geneticSampleID）。大型底栖无脊椎动物样本每年在可涉水溪流、河流及湖泊样地开展三次采集，对应水生生物学调查窗口期，大致覆盖春季、夏季与秋季。样本采集将根据生境类型选用适配的采样器具，包括Surber采样器（Surber sampler）、Hess采样器（Hess sampler）、手持采泥器（hand corer）、改良式踢网（modified kicknet）、D形扫网（D-frame sweep）以及小型波拿采泥器（petite ponar sampler）。大型无脊椎动物采样采用基于百分比的大生境采样方案：在可涉水溪流中，采样将在每处1公里长的溪流样地内的两种优势生境类型（如急流、缓流、深潭、阶梯深潭）中开展；在湖泊中，采样将在浮标、入水口及出水口传感器附近采用小型波拿采泥器进行，沿岸带则使用D形扫网采集；在河流中，采样将在浮标及另外两处深水点位采用小型波拿采泥器开展，沿岸带则使用D形扫网或大型木质碎屑采样器（large-woody debris sampler）采集。野外采样规程因生境与基质类型而异，但所有样本均采集自各生境中天然基质的表面。样本在野外以乙醇保存，随后送至区域支持设施更换保存液，再进行寄送。第二批次（夏季）的DNA样本将送往外部实验室开展元条形码测序（metabarcoding）；第一批次（春季）与第三批次（秋季）的未分拣批量DNA样本将被存档，以备未来研究使用。未分拣的大型底栖无脊椎动物批量样本以70%-95%乙醇保存，并在17-18℃的温湿度受控环境中存档。如需了解采样规程及NEON相关数据产品，请参阅下方相关链接。