Water column metagenomes from two Canadian Boreal Shield lakes. freshwater metagenome

Following initial 16S rRNA gene amplicon sequencing (BioProject PRJNA354806), six selected genomic DNA samples collected from the anoxic zones of Lakes 227 and 442 (International Institute for Sustainable Development Experimental Lakes Area, AKA IISD-ELA; near Kenora, Ontario, Canada) were re-analyzed by metagenomic sequencing. The goal of the present study was to predict the metabolisms of distinct populations of anoxygenic photoautotrophs in the lake anoxic zones via genome-resolved metagenomics. More broadly, it is of interest to analyze the biogeochemical cycling potential of microbial populations in the lake anoxic zones, especially with respect to their potential involvement in the iron cycle, given the ferruginous nature of the lakes.

在最初的16S rRNA基因扩增子测序（生物项目号PRJNA354806）完成后，从加拿大安大略省基诺拉附近的国际可持续发展研究所实验湖区（简称IISD-ELA）的227号湖与442号湖缺氧区域采集的6份精选基因组DNA样本，通过宏基因组测序进行了重新分析。本研究的核心目标是借助基因组解析宏基因组学，预测湖泊缺氧区域中不同类群的不产氧光合自养微生物的代谢模式。从更广泛的研究视角出发，鉴于这些湖泊的铁质化特性，本研究同时旨在解析湖泊缺氧区域微生物种群的生物地球化学循环潜力，尤其是其在铁循环中潜在的参与作用。