Comparative gene expression profiles of isolated islets of GK and Wistar rats using Affymetrix Rat Clariom-S data.

Goto-Kakizaki (GK) rat is a spontaneous nonobese T2DM model which has clinical and pathological similarities with Japanese patient and was often used to study T2DM and its complications. GK rat and Wistar rat exhibited different islet functions and phenotypes such as insulin secretion deficiency and mild insulin resistance. We used microarrays to unravel the molecular basis underlying these alterations of endocrine cell componentsby use of isolated pancreatic islets of GK and Wistar rats at the age of 29 weeks.GK大鼠（Goto-Kakizaki rat）是一种自发性非肥胖2型糖尿病（Type 2 Diabetes Mellitus，T2DM）模型，其临床与病理特征与日本糖尿病患者高度契合，常被应用于2型糖尿病及其并发症的相关研究。GK大鼠与Wistar大鼠的胰岛功能及表型存在显著差异，具体表现为胰岛素分泌缺陷与轻度胰岛素抵抗等特征。本研究以29周龄GK大鼠与Wistar大鼠的分离胰岛为实验材料，采用基因芯片技术，解析其内分泌细胞组分改变背后的分子机制。