Genome-wide mapping of CASZ1b binding sites and investigate its effect on chromatin status in SMS-CTR cells [CTRtetCASZ1b]. Genome-wide mapping of CASZ1b binding sites and investigate its effect on chromatin status in SMS-CTR cells [CTRtetCASZ1b]

In SMS-CTR cells, we identified genomewide binding sites of CASZ1b. The overexpression of CASZ1b in SMS-CTR cells led to a regional epigenetic modification. Overall design: In the CASZ1b stably transfected SMS-CTR cells (CTRtetCASZ1b), CASZ1b expression is Doxcycline (Dox) inducible . The CTRtetCASZ1b cells were treated with or without Dox for 48 hr, and ChIP seq was performed using anti-CASZ1 antibody and antibodies of histone markers and RNA Pol II.

本研究在SMS-CTR细胞中鉴定了CASZ1b的全基因组结合位点。在SMS-CTR细胞中过表达CASZ1b，可引发区域性表观遗传修饰。实验整体设计：在稳定转染CASZ1b的SMS-CTR细胞（CTRtetCASZ1b）中，CASZ1b的表达受多西环素（Doxcycline，简称Dox）诱导。将CTRtetCASZ1b细胞分别用含Dox与不含Dox的培养基处理48小时后，采用抗CASZ1抗体、组蛋白标记物抗体以及RNA聚合酶II（RNA Pol II）抗体开展染色质免疫沉淀测序（ChIP-seq）实验。