Global changes in gene expression in primary keratinocytes after KLF9 overexpression. Homo sapiens

To identify putative target genes of the transcription factor Klf9 in primary keratinocytes we over-expressed Klf9 in these cells using a lentiviral delivery system. We detected several hundred genes that show differential expression levels following ectopic Klf9 expression including several genes that are involved in proliferation and cell cycle control. These results allow insights into the mechanisms by which Klf9 regulates proliferation in primary keratinocytes. Overall design: Primary human neonatal foreskin keratinocytes were infected with equal viral titers of a GFP and KLF9 expression construct, respectively. Cells were harvested 2,4 and 7 days after infection and total RNA was used to perform whole genome microarray analysis.

为鉴定转录因子Klf9在原代角质形成细胞（primary keratinocytes）中的推定靶基因，我们采用慢病毒递送系统（lentiviral delivery system）在该类细胞中过表达Klf9。我们检测到数百个在异位表达Klf9后呈现差异表达的基因，其中包含多个参与细胞增殖与细胞周期调控的基因。本研究结果可为解析Klf9调控原代角质形成细胞增殖的分子机制提供理论依据。实验设计概述：将原代人新生儿包皮角质形成细胞分别以等量病毒滴度感染携带绿色荧光蛋白（GFP）与KLF9表达构建体的病毒载体。分别于感染后2、4及7天收集细胞，提取总RNA并开展全基因组微阵列分析（whole genome microarray analysis）。