Fecal and stomach microbiomes of invasive black rats in Hawaii: rodent metagenome Raw sequence reads

We couple microbial forensics with molecular dietary analysis to quantify species interactions and identify scavenging by invasive rats on native and introduced birds in Hawaii. We characterized the gut microbiome (stomach and fecal samples) of invasive black rats from 17 kipuka in Hawaii using 16S rRNA high-throughput sequencing. Those data are uploaded here. We identified 17 bacterial taxa that were (i) detected only in the rats that had consumed birds (n = 20/51) and (ii) were indicative of decaying tissue in the chicken model. The chicken model was used to characterize bacterial signatures of bird carcass decay by conducting 16S rRNA high-throughput sequencing on chicken (Gallus gallus domesticus) tissues collected over a 11-day decomposition study. Those data were uploaded as a separate submission.

我们将微生物法医（microbial forensics）与分子饮食分析相结合，以量化物种间相互作用，并识别夏威夷地区入侵鼠对本土及外来鸟类的食腐行为。我们采用16S rRNA高通量测序技术，对来自夏威夷17处基普卡（kipuka）的入侵黑鼠的肠道微生物组（胃内容物与粪便样本）进行了表征分析，相关数据已上传至本数据集。我们共鉴定出17个细菌分类群，其满足两项特征：一是仅在食用过鸟类的大鼠（n=20/51）中被检测到；二是可作为鸡模型中组织腐败的指示物。为表征鸟类尸体腐败的细菌特征，我们构建了鸡模型：通过对为期11天腐烂实验中采集的家鸡（Gallus gallus domesticus）组织样本进行16S rRNA高通量测序，完成了相关表征工作。该模型实验的相关数据已作为独立提交项上传。