m6A methyltransferase Mettl3 is essential for neuronal homeostasis and sleep via modulating Npy in mice

To unravel the underlying mechanisms how neuronal Mettl3 loss induces the activation of microglia, we performed RNA-sequencing (RNA-seq) with the thalamic tissues of 2- and 4-month-old Ctrl and cKO mice, respectively. Overall design: we performed bulk RNA sequencing (RNA-seq) with both ctrl and cKO mice brain in 2month and 4month to reveals the altered gene expression relating to microglia

为阐明神经元Mettl3缺失诱导小胶质细胞活化的潜在分子机制，我们分别对2月龄和4月龄对照组（Ctrl）及条件性敲除（cKO）小鼠的丘脑组织开展了RNA测序（RNA-sequencing，RNA-seq）。整体实验设计：我们对2月龄与4月龄的对照组及条件性敲除小鼠的脑组织进行批量RNA测序（bulk RNA-sequencing，bulk RNA-seq），以揭示与小胶质细胞相关的差异基因表达变化。