Expression data from CD8alpha positive dendritic cells. Mus musculus

We screened a number of interferon inducible genes that may be involved in impeding HBV replication and found an anti-HBV activity in ISG20. ISG20 is an IFN-inducible 3’- to 5’-exonuclease, that degrades DNA and RNA and reduces antigen production in hepatocyte-derived cells A range of candidate genes whose expression was dependent on type I IFN stimulation were identified by gene arrays Overall design: Total RNA from CD8α+ DCs was extracted from mouse spleens 8h after polyI:C (50 μg) stimulation by RNeasyTM kit. Total RNA (10 μg) from each sample was used to prepare cRNA. Gene expression was analyzed with GeneChipTM

本研究筛选了一批可能参与抑制乙型肝炎病毒（Hepatitis B Virus, HBV）复制的干扰素诱导基因，并在ISG20中发现了抗HBV活性。ISG20是一种干扰素（Interferon, IFN）诱导的3'→5'外切核酸酶，可降解DNA与RNA，并降低肝细胞来源细胞中的抗原生成水平。本研究通过基因芯片技术，鉴定出一批表达依赖于I型干扰素（Type I Interferon, I型IFN）刺激的候选基因。总实验设计：采用RNeasyTM试剂盒，从经聚肌胞苷酸（polyinosinic-polycytidylic acid, polyI:C，50 μg）刺激8小时的小鼠脾脏中提取CD8α阳性树突状细胞（CD8α+ dendritic cells, CD8α+ DCs）的总RNA；每份样本取10 μg总RNA用于制备互补RNA（complementary RNA, cRNA）；随后采用GeneChipTM芯片开展基因表达分析。