Example of the two-way matrix-pooling system used to identify T cell reactive peptides from pools representing CSFV proteins.

*The 110 overlapping 15mer peptides (#1-110) representing CSFV NS2 were pooled using a matrix system so that each peptide was uniquely represented in two matrix-pools (Pools A-U).
#Matrix pools B, I, O and P stimulated CD8 T cell reactivity from Pig AN7 and so NS2 peptides #35, 42, 46 and 53 were identified for further testing.

* 本研究采用矩阵混池策略，对覆盖猪瘟病毒（CSFV）非结构蛋白2（NS2）的110条重叠15聚体肽（编号1-110）进行混样，每条肽段仅在编号为A至U的两个矩阵池（Pools A-U）中各出现一次。
# 矩阵池B、I、O及P可激活猪AN7的CD8+ T细胞产生免疫应答，因此筛选出NS2蛋白来源的肽段#35、42、46与53用于后续实验验证。