Gene expression data of different SSc subsets

We here used whole blood gene expression profiling to differentiate SSc patients from healthy controls (HC) and to identify a specific gene expression and predictive genes for SSc-overlap syndromes. For each collection, 2,5ml of peripheral whole blood of 150 SSc patients and 40 HC was drawn into 2 PAXGene Blood RNA tubes of PreAnalytiX (Qiagen, BD Company, Hilden, Germany). Gene expression analysis was performed using the Affymetrix Human Genome U219 Chip.

本研究采用全血基因表达谱分析技术，以区分系统性硬化症（Systemic Sclerosis, SSc）患者与健康对照（Healthy Controls, HC），并鉴定出针对SSc重叠综合征的特征性基因表达特征与预测基因。本研究共纳入150名SSc患者与40名健康对照，每例受试者均采集2.5mL外周全血，分装至2支由PreAnalytiX（Qiagen、BD Company，德国希尔德市）生产的PAXgene血液RNA采集管（PAXGene Blood RNA tubes）中。基因表达分析采用Affymetrix Human Genome U219 芯片完成。