Genome-wide High Resolution Mapping of Mitotic DNA Synthesis Sites and Common Fragile Sites by Direct Sequencing [MiDA-seq]. Genome-wide High Resolution Mapping of Mitotic DNA Synthesis Sites and Common Fragile Sites by Direct Sequencing [MiDA-seq]

Common fragile sites (CFSs) are genomic loci prone to the formation of breaks or gaps on metaphase chromosomes. Here, we seek to map human CFSs with high resolution on a genome-wide scale by sequencing the sites of mitotic DNA synthesis (MiDASeq) that are specific for CFSs. We generated a nucleotide-resolution atlas of MiDAS sites (MDSs) that covered most of know CFSs, and comprehensively analyzed their sequence characteristics and genomic features. Overall design: Sequencing the sites of mitotic DNA synthesis (MiDASeq) that are specific for CFSs in three cell lines.

常见脆性位点（Common fragile sites, CFSs）是一类易于在中期染色体上形成断裂或间隙的基因组位点。本研究旨在通过靶向常见脆性位点特异性的有丝分裂DNA合成测序（mitotic DNA synthesis sequencing, MiDASeq）技术，在全基因组范围内以高分辨率绘制人类常见脆性位点图谱。我们构建了覆盖绝大多数已报道常见脆性位点的核苷酸分辨率有丝分裂DNA合成位点（MiDAS sites, MDSs）图谱，并对其序列特征与基因组学特征开展了全面分析。本研究整体实验设计为：在三种细胞系中，对针对常见脆性位点特异性的有丝分裂DNA合成位点进行MiDASeq测序。