Gene expression profiling in dentate granule cells from patients with mesial temporal lobe epilepsy with or without hippocampal sclerosis. Homo sapiens

Hippocampal sclerosis (HS) is the most common neuropathological finding of medically intractable cases of mesial temporal lobe epilepsy (MTLE), the most common form of partial epilepsy. Within the dentate gyrus, HS may be associated with granule cell dispersion and aberrant mossy fiber sprouting, and these pathological changes are accompanied by a range of molecular changes. In this study, we analyzed the gene expression profiles of dentate granule cells of MTLE patients with and without HS to show that next-generation sequencing methods can produce interpretable genomic data from RNA collected from small homogenous cell populations and to shed light on the transcriptional changes associated with HS. Overall design: 12 samples of dentate granule cells from patients with mesial tempora lobe epilepsy, 5 with hippocampal sclerosis and 7 without hippocampal sclerosis. 10 samples had replicates.

海马硬化（Hippocampal sclerosis, HS）是药物难治性内侧颞叶癫痫（mesial temporal lobe epilepsy, MTLE）患者最常见的神经病理学特征，而内侧颞叶癫痫亦是最为常见的部分性癫痫类型。在齿状回中，海马硬化常伴随颗粒细胞散在与异常苔藓纤维出芽，此类病理改变往往伴随着一系列分子层面的异常变化。本研究针对伴与不伴海马硬化的内侧颞叶癫痫患者的齿状回颗粒细胞开展基因表达谱分析，旨在证实新一代测序（next-generation sequencing）技术可从少量均质细胞群体提取的RNA中获取可解读的基因组数据，并阐明与海马硬化相关的转录组改变。实验设计：共纳入12例内侧颞叶癫痫患者的齿状回颗粒细胞样本，其中5例伴海马硬化，7例不伴海马硬化；其中10份样本设有生物学重复。