Drosophila melanogaster Targeted Locus (Loci)

We report the m6dA modification on the Drosophila genome. We collected ovary genomic DNA from 2-day wild-type and DMAD mutant files and performed DNA-immunoprecipitation(DNA-IP) experiments using anti-m6dA antibody, and the generated DNA library, which was subjected to a high-throughput sequencing analysis. In this assay, the IgG-immunoprecipited DNA from the same amount of wild-type ovaries was used as the control, and the high-throughput sequencing resulted in a range of approximately 4.2 to 5.5 million reads. In sum, we identified 161 and 491 peaks from wild-type and DMAD mutant samples. Importantly, m6dA is mainly utilized to modify the transposon sequence on the chromosomes. To link the m6dA modification with transposon expression, we employed wild-type and DMAD mutant samples to perform RNA-seq analysis.

本研究报道了果蝇基因组上的N6-甲基脱氧腺苷（m6dA）修饰。我们从2日龄野生型与DMAD突变体果蝇的卵巢中提取基因组DNA，使用抗m6dA抗体开展DNA免疫沉淀（DNA-immunoprecipitation, DNA-IP）实验，构建所得DNA文库随后进行高通量测序分析。本实验中，我们以等量野生型卵巢的IgG免疫沉淀DNA作为对照，本次高通量测序共获得约420万至550万条读段。综上，我们分别从野生型和DMAD突变体样本中鉴定出161个和491个富集峰。值得注意的是，m6dA修饰主要靶向修饰染色体上的转座子序列。为了阐明m6dA修饰与转座子表达之间的关联，我们利用野生型和DMAD突变体样本开展了RNA测序（RNA-seq）分析。