Loss of ANCO1 repression of AIB1/YAP drives breast cancer progression. Loss of ANCO1 repression of AIB1/YAP drives breast cancer progression

We report the role of AIB1 in regulated YAP driven transcription and repression in normal mammary epithelial cells (MCF10A). We generated six cell lines by sequential infection and selection and extracted RNA for sequencing. Data was aligned to Hg38 with STAR and processed with EdgeR. Overall design: Examination of RNA-seq of 6 cell lines in triplicate

本研究报道了乳腺癌扩增基因1（AIB1）在正常乳腺上皮细胞MCF10A中调控Yes相关蛋白（YAP）介导的转录激活与转录抑制过程中的作用。我们通过连续感染与筛选构建了六株细胞系，并提取RNA进行测序。测序数据通过STAR工具比对至人类参考基因组Hg38，并使用EdgeR软件完成数据处理。整体实验设计：对6株细胞系的RNA测序样本进行三次生物学重复实验。