DataSheet_1_Metabolomics Analysis Discovers Estrogen Altering Cell Proliferation via the Pentose Phosphate Pathway in Infertility Patient Endometria.docx

Estrogen therapy is widely used as a supplementary treatment after hysteroscopy for female infertility patients owing to its protective function that improves endometrial regeneration and menstruation, inhibits recurrent adhesions, and improves subsequent conception rate. The endometrial protective function of such estrogen administration pre-surgery is still controversial. In the current study, 12 infertility patients were enrolled, who were treated with estrogen before hysteroscopy surgery. Using cutting-edge metabolomic analysis, we observed alterations in the pentose phosphate pathway (PPP) intermediates of the patient’s endometrial tissues. Furthermore, using Ishikawa endometrial cells, we validated our clinical discovery and identified estrogen–ESR–G6PD–PPP axial function, which promotes estrogen-induced cell proliferation.

雌激素疗法可改善子宫内膜再生与月经状态、抑制复发性粘连形成并提升后续受孕率，因此被广泛应用于女性不孕患者宫腔镜术后的辅助治疗。而术前给予雌激素的子宫内膜保护作用仍存在争议。本研究纳入12名于宫腔镜术前接受雌激素治疗的不孕患者，通过前沿代谢组学分析，我们观察到患者子宫内膜组织中磷酸戊糖途径（PPP）中间代谢物的变化。此外，我们利用石川子宫内膜细胞（Ishikawa endometrial cells）验证了这一临床发现，并明确了雌激素–ESR–G6PD–PPP轴的功能，其可促进雌激素诱导的细胞增殖。