Long-lasting effect of jasmonic acid (JA) seedling treatment on the transcriptional response to JA challenge of five-week-old Arabidopsis thaliana (ecotype Col-0).

Treatment of Arabidopsis thaliana (ecotype Col-0) seedlings with jasmonic acid (JA) elicits long-term induced resistance (IR) against the chewing herbivore Spodoptera littoralis. We used RNA-seq to profile the transcriptional changes associated with this long-lasting JA-IR. Overall design: mRNA profiles for the leaves of 5-week-old plants treated 4 hours previously with water or JA. Plants were from 2-week-old seedlings treated with water or JA. A total of 16 RNA-seq samples, 4 biological replicates for each of the 4 treatment groups.

对生态型Col-0的拟南芥（Arabidopsis thaliana）幼苗施加茉莉酸（jasmonic acid, JA）处理，可引发针对咀嚼式食草动物斜纹夜蛾（Spodoptera littoralis）的长期诱导抗性（induced resistance, IR）。本研究采用RNA测序（RNA-seq）技术，对与该持久JA诱导抗性相关的转录组变化进行分析。实验总体设计：测序对象为5周龄植株的叶片，这些植株源自经清水或JA处理的2周龄幼苗，且在取样前4小时接受了清水或JA处理。本实验共设置4个处理组，每组包含4个生物学重复，总计16个RNA-seq样本。