The lineage-specific transcriptional regulator, CDX2, navigates a dynamic chromatin landscape to control distinct stages of intestinal development

Important lineage regulators, such as the intestinal lineage regulator CDX2, can function over the timespan of intestinal development. The consequences of CDX2 knockout in the embryo versus in the adult are quite distinct. The data below provide a rationale for these divergent transcription factor functions in distinct developmental contexts, with unique binding patterns of CDX2 observed via ChIP-seq and unique gene regulatory targets defined via RNA-seq. The dynamic gene regulatory program controlled by CDX2 is conserved in mice and humans. Overall design: CDX2 ChIP-seq and RNA-seq experiments were conducted at various stages of mouse or human iPSC-derived tissues or primary adult human tissues.重要的谱系调控因子，例如肠道谱系调控因子CDX2，可在肠道发育的整个时序进程中发挥功能。CDX2基因敲除在胚胎与成年个体中所引发的功能效应存在显著差异。下文所述数据集阐释了该转录因子在不同发育情境下功能分化的分子机制：通过染色质免疫共沉淀测序（ChIP-seq）鉴定得到CDX2的特异性结合模式，通过转录组测序（RNA-seq）确定其独特的基因调控靶标。CDX2所调控的动态基因调控程序在小鼠与人类中具有进化保守性。实验整体设计：分别在小鼠或人类诱导多能干细胞（iPSC）分化来源组织的不同发育阶段，以及成人原代组织中开展CDX2的ChIP-seq与RNA-seq实验。