Additional file 2: of A survey of transcriptome complexity using PacBio single-molecule real-time analysis combined with Illumina RNA sequencing for a better understanding of ricinoleic acid biosynthesis in Ricinus communis

Figure S1. Correlation thermograms between 18 samples from Illumina RNA sequencing. Figure S2. ROI read length distribution of each size (1–2 k, 2-3 k and 3-6 k) of bins in the cDNA database. Figure S3. GO classification according to cellular component, molecular function and biological process of differentially expressed genes. Strain 349 vs. strain 1115 at 7 DAF (A), 14 DAF (B), and 21 DAF (C). Figure S4. KEGG classification and Pathway enrichment statistics of differentially expressed genes. (A) KEGG classification between strains 349 and 1115 at 7 DAF. (B) KEGG classification between 349 and 1115 at 14 DAF. (C) KEGG classification between 349 and 1115 at 21 DAF. (D) Pathway enrichment statistics between 349 and 1115 at 7 DAF. (E) Pathway enrichment statistics between 349 and 1115 at 14 DAF. (F) Pathway enrichment statistics between 349 and 1115 at 14 DAF. Figure S5. COG function classification of differentially expressed genes. (A) 349 vs. 1115 at 7 DAF. (B) 349 vs. 1115 at 14 DAF. (C) 349 vs. 1115 at 21 DAF. (ZIP 29130 kb)

补充图S1. 基于Illumina RNA测序的18份样本间的相关热图。补充图S2. cDNA数据库中各分箱（bins）对应不同长度区间（1–2 k、2–3 k及3–6 k）的感兴趣区域（Region of Interest，ROI）读取长度分布。补充图S3. 差异表达基因的GO分类注释，按细胞组分、分子功能与生物过程三大类别进行分类，分别对应花后7天（7 DAF，Days After Flowering，A）、14天（14 DAF，B）及21天（21 DAF，C）时菌株349与菌株1115的比较组。补充图S4. 差异表达基因的KEGG分类及通路富集统计：(A) 花后7天时菌株349与1115的KEGG分类；(B) 花后14天时菌株349与1115的KEGG分类；(C) 花后21天时菌株349与1115的KEGG分类；(D) 花后7天时菌株349与1115的通路富集统计；(E) 花后14天时菌株349与1115的通路富集统计；(F) 花后14天时菌株349与1115的通路富集统计。补充图S5. 差异表达基因的COG功能分类：(A) 花后7天时菌株349与1115的比较组；(B) 花后14天时菌株349与1115的比较组；(C) 花后21天时菌株349与1115的比较组。（压缩包大小：29130 kb）