The effects of transcriptional activator engineering on the transcriptome of Penicillium oxalicum. The effects of transcriptional activator engineering on the transcriptome of Penicillium oxalicum

The filamentous fungus Penicillium oxalicum can secret various enzymes for efficient saccharification of plant biomass materials. Expression of the constitutively active forms of transcriptional activators ClrB, XlnR and AraR could trigger the production of different sets of lignocellulolytic enzymes. Here, the transcriptomes of the three engineered strains were compared with that of wild type in the medium without carbon source. Overall design: A total of 12 samples were analyzed, which include biological triplicates. The wild-type strain 114-2 was used as a control.

草酸青霉（Penicillium oxalicum）可分泌多种酶，实现植物生物质材料的高效糖化。转录激活因子ClrB、XlnR与AraR的组成型激活形式，可诱导产生不同类别的木质纤维素降解酶。本研究将三株工程菌株的转录组，与无碳源培养基中培养的野生型菌株转录组进行了对比分析。实验设计概述：本次分析共纳入12份样本，所有样本均设置生物学重复三次；实验以野生型菌株114-2作为对照。