Methylation data of Chinese Spring leaves

We used enzymatic methyl-sequencing (EM-Seq) to detect DNA methylation patterns at single-base resolution in hexaploid wheat. Genomic DNA was extracted from the flag leaves of Chinese Spring wheat, and libraries were prepared using TET2+APOBEC3A enzyme and PCR amplification. Sequencing was performed at an average depth of 14x. This results demonstrate that the EM-Seq approach is cost-effective and accurate for studying DNA methylation in plants with large and complex genomes.

我们采用酶促甲基化测序（enzymatic methyl-sequencing，EM-Seq）在单碱基分辨率水平检测六倍体小麦的DNA甲基化模式。从中国春小麦的旗叶中提取基因组DNA，使用TET2+APOBEC3A酶及PCR扩增技术制备测序文库，测序平均深度达14×。本研究结果表明，EM-Seq方法对于研究大型复杂基因组植物的DNA甲基化具有成本效益高且准确的优势。