Phomopsis mahothocarpi strain:GZU-Y2 Genome sequencing. Phomopsis mahothocarpi strain:GZU-Y2

To enhance comprehension of the characteristics and pathogenic mechanism of this pathogen, and to facilitate the development of better methods for preventing and controlling leaf blight disease, we sequenced and analyzed the entire genome of this strain. We used the Pacbio Sequel II sequencing platform, one of the most advanced triple sequencing platforms, to perform whole-genome CCS sequencing and genome assembly. The resulting high-quality genome sequence of Diaporthe mahothocarpus GZU-Y2 was analyzed. Genome prediction, functional annotation, and sequencing were performed to obtain the complete genome sequence of Diaporthe mahothocarpus GZU-Y2 and to investigate its mechanism of action on Camellia oleifera. The fungal genome sequence size was 58.97Mb (N50), with a GC content of 50.65% and allele size greater than 1 Mb. The strain was assembled to achieve a complete genome length of 58,973,678bp. A total of 15,918 genes were predicted and annotated using multiple bioinformatics databases. The repetitive sequences accounted for approximately 3.22% of the genome. Molecular functions, cellular components and biological processes of predicted genes are mapped to the genome. The genome assembly, component analyses, and annotation resources presented in this paper will aid in the comprehension of its pathogenesis, mechanisms of fungal infection, and studies of pathogen-host interactions.

为加深对该病原菌的特性与致病机制的理解，推动叶枯病防控方法的优化研发，我们对该菌株开展了全基因组测序与分析。本研究采用当前最先进的三代测序平台之一的PacBio Sequel II测序平台，进行全基因组CCS（环形一致性测序，Circular Consensus Sequencing）测序及基因组组装，对获得的高质量间座壳属菌株Diaporthe mahothocarpus GZU-Y2全基因组序列进行解析。通过基因组预测、功能注释与多组生物信息学分析，获取该菌株的完整基因组序列，以探究其对油茶（Camellia oleifera）的致病机制。该真菌基因组的N50值为58.97Mb，GC含量为50.65%，等位基因片段长度大于1 Mb；该菌株组装得到的完整基因组总长为58,973,678bp。通过多类生物信息学数据库，共预测并注释得到15918个编码基因，重复序列约占基因组总长的3.22%。预测基因的分子功能、细胞组分与生物学过程均已注释至该基因组。本文所提供的基因组组装、组分分析及注释资源，将为解析该病原菌的致病机理、真菌侵染机制以及开展病原菌-宿主互作研究提供重要支撑。