Pathogenic mechanisms implied by plasma exosomal miRNA profile of preeclampsia with severe features

Preeclampsia is a common complication of pregnancy that affects 4-5% of pregnant women around the world. At present, there is a lack of early identification of high-risk patients of preeclampsia in clinical practice, which restricts the development of disease prevention and treatment. Previous studies have indicated that plasma exosomal miRNAs in pregnant women could serve as biomarkers of preeclampsia, but few is focused on exosomal miRNAs from preeclampsia pregnancy with severe features(sPE). Therefore, we detected and compared the plasma exosomal miRNA profiles between normal pregancy and sPE to explore potential biomarkers and pathogenic mechanisms of sPE. Overall design: Blood samples were collected from pregnant women in control group (normotensive pregnant women, n=3) and sPE group (patients with sPE, n=3). After exosome isolation and RNA extraction, exosomal miRNAs were profiled using next generation seqeuencing.

子痫前期（Preeclampsia）是全球范围内影响4%~5%孕妇的常见妊娠并发症。目前临床实践中仍缺乏对子痫前期高危患者的早期识别手段，这一现状制约了该疾病防治工作的开展。既往研究表明，孕妇血浆来源外泌体（exosome）的微小核糖核酸（microRNA，miRNA）可作为子痫前期的生物标志物，但针对伴重症特征的子痫前期（severe preeclampsia，sPE）孕妇来源外泌体miRNA的相关研究仍较为匮乏。因此，本研究通过检测并对比正常妊娠与sPE孕妇的血浆外泌体miRNA表达谱，以期探索sPE潜在的生物标志物及致病机制。

实验设计概述：采集对照组（血压正常孕妇，n=3）与sPE组（sPE患者，n=3）的外周血样本。完成外泌体分离与RNA提取后，采用下一代测序技术对样本中的外泌体miRNA进行表达谱分析。