Egg production, hatching, faecal pellet production of Calanus finmarchicus in the North Atlantic

The samples were collected using a T-80 net (375 µm mesh size) equipped with a non-filtering cod-end in the North Atlantic during the G.O. Sars Trans-Atlantic cruise in 2013. Within 15-30 minutes after the recovery, 20 Calanus finmarchicus females were sorted out under microscope in ice chilled petri dishes and incubated individually in 600 ml polycarbonate culture bottles resulting in 20 replicate measurements. The bottles were filled with 50 µm screened seawater originated from 6 m water depth. The samples were incubated upright in thermoroom for 24 hours at the surface temperature (3°C). After the samples had been filtered (40 µm filter), female prosome length, egg as well as pellet abundance were determined. Subsequently, eggs from six females were incubated in petri dishes at 5°C. After 4 days, the number of nauplii and eggs were counted in order to calculate hatching success.

本数据集的样本于2013年搭载G.O. Sars号跨大西洋科考航次，在北大西洋海域使用配备非过滤型拖网囊网（cod-end）的T-80网（网目尺寸375 µm）采集获得。样本回收后的15~30分钟内，科研人员在冰浴培养皿中通过显微镜分拣出20只飞马哲水蚤（Calanus finmarchicus）雌性成体，并将其单独置于600 mL聚碳酸酯培养瓶中进行培养，最终得到20组重复测量数据。培养瓶内注入取自6米水深、经50 µm筛网过滤的海水。样本于恒温室内以直立姿态培养24小时，培养温度为表层海水温度（3°C）。待样本经40 µm滤器过滤后，测定雌性成体前体长度（prosome length）、卵粒丰度及粪粒丰度。随后，选取6只雌性成体所产的卵粒置于培养皿中，在5°C条件下培养。培养4天后，统计无节幼体（nauplii）与未孵化卵粒的数量，以此计算孵化成功率。