Human Microbiome Study-ESTEEM

The overarching aim of the HMS-ESTEEM study was to describe the role that the urinary microbiome plays in disorders of the lower genitourinary system, namely, the female genitourinary microbiome. The primary aim of the study evaluated whether the urinary microbiome differs between women with mixed urinary incontinence (MUI) and unaffected controls. There were 212 women (128 affected and 84 controls) who participated in the study and had catheterized urine specimens, vaginal specimens sent for culture, and DNA analysis. From the catheterized urine samples obtained, v4–6 regions of the 16S rRNA gene were sequenced to identify bacteria. Women with MUI and controls did not differ in overall Lactobacillus predominance. In younger women, urinary bacterial community-types differentiated MUI from controls.

HMS-ESTEEM研究的总体研究目标为阐明泌尿微生物组（urinary microbiome）在下泌尿生殖系统疾病中的作用，具体聚焦于女性泌尿生殖微生物组。本研究的主要目标为评估混合性尿失禁（mixed urinary incontinence, MUI）女性与健康对照者的泌尿微生物组是否存在差异。本研究共纳入212名女性受试者（其中128名混合性尿失禁患者，84名健康对照），所有受试者均留取导尿尿液标本、送检阴道培养标本并完成DNA分析。从获取的导尿尿液样本中，对16S rRNA基因的v4–6可变区进行测序以鉴定细菌物种。混合性尿失禁患者与健康对照者的整体乳杆菌（Lactobacillus）主导比例无显著差异；在年轻女性群体中，尿液细菌群落型可有效区分混合性尿失禁患者与健康对照者。