Commitment to the phosphate starvation response after replenishment of phosphate. Saccharomyces cerevisiae

Diploid wild type cells in a chemostat, in different times after changing the feeding medium from low to high Pi. Overall design: Diploid cells were also grown in a chemostat at a dilution rate of 0.3[1/hr] and phosphate concentration in the feeding vessel of 0.3mM Pi. Cells were allowed to reach steady state and then feeding medium was replaced to 7.3mM Pi. Samples from the chemostat were harvested in different times after the transfer (t= 0.35, 2, 5, and 10 days). Total RNA was extracted using MasterPure™ Kit (Epicentre). The samples were amplified, labeled, hybridized to yeast dual color expression microarrays and scanned, all using standard Agilent protocols, reagents, and instruments. The scanned images were analyzed using SpotReader software (Niles Scientific).

本数据集的研究对象为恒化器培养的二倍体野生型细胞，采集了将饲喂培养基由低磷（Pi）切换为高磷（Pi）后的多个时间点样本。实验设计概述：将二倍体细胞以0.3[1/小时]的稀释速率在恒化器中培养，饲喂容器内的磷酸盐浓度设置为0.3mM Pi。待细胞达到稳态后，将饲喂培养基更换为含7.3mM Pi的培养基。在培养基切换后的不同时间点（t=0.35、2、5和10天）收集恒化器内的细胞样本。采用MasterPure™试剂盒（Epicentre公司）提取总RNA。后续的样本扩增、标记、与酵母双色表达微阵列杂交及扫描步骤，均严格遵循安捷伦（Agilent）标准协议、试剂及仪器流程完成。扫描得到的图像使用SpotReader软件（Niles Scientific公司）进行分析。