Dataset supporting Figure 6 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"
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https://plus.figshare.com/articles/dataset/Dataset_supporting_Figure_6_in_Pan-cortical_2-photon_mesoscopic_imaging_and_neurobehavioral_alignment_in_awake_behaving_mice_/25114824
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Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from the same side mount mouse as in Figure 5, but corresponding to Figure 6. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data. Also contains DeepLabCut and BSOiD movies and analysis files corresponding to Figure 6 and to three other sessions from the same side mount mouse used, along with the target session, to train the BSOiD model used to fit the target session.Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in <i>header.csv). </i>Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.Please contact the authors for any additional clarifications as needed.See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513
本数据集包含与图5所用为同一只侧置安装小鼠的单次单光子宽场成像实验、单次Thorlabs(索雷博)双光子介观成像会话数据,但对应图6。
所包含的文件涵盖成像数据、行为学数据,以及用于整合神经行为学数据的Python脚本。
此外还包含对应图6,以及该侧置安装小鼠另外三次成像会话(与目标会话一同用于训练适配目标会话的BSOiD(BSOiD)模型)的DeepLabCut(DeepLabCut)与BSOiD(BSOiD)行为分析视频及分析文件。
会话名称遵循以下格式:"mouse#_bigEndianDate_cage#_info_session#_attempt#"。
原始介观成像数据以ScanImage(ScanImage)渲染格式存储,为单张大尺寸TIFF文件,命名规则为"filename_2D.tiff"。
小鼠面部与身体摄像头图像以独立或拼接的.avi视频文件形式提供,行为学数据同时包含Spike2(Spike2)格式(.smrx)文件与导出的Matlab(Matlab)数据文件(.mat)。
所有数据均以LabVIEW(LabVIEW)发出的首个帧时钟触发信号为基准完成对齐:该信号连同所有其他帧时钟事件一同被Spike2(Spike2)记录,对应双光子电影的第一帧、右侧面部/身体视频的第一帧以及Spike2(Spike2)原始数据。
双光子触发信号有时会在Spike2(Spike2)中出现记录错误(通常我们会同时将其记录为事件与波形),但所有情况下均可从ScanImage(ScanImage)的TIFF元数据中导出时间戳,即以"header.csv"结尾的CSV文件。
会话起始时间戳同样从ScanImage(ScanImage)的TIFF元数据中导出,以"_starttime.txt"结尾的TXT文件形式存储。
预处理后的Python格式数据存储于各类命名的NPY文件中,各文件包含分析所需的不同变量子集。对于每个成像会话,包含字符串"standard_frames"的NPY文件包含最完整的最终阶段预处理神经行为学数据(采用可导出至NWB(NWB)的组合DataFrame格式),涵盖CCF/MMM对齐信息。包含字符串"nb_dump"的文件则包含大量用于额外预处理的辅助变量。
此外还包含各类图像文件(TIFF、PNG)以及Excel工作表(XLSX、CSV),用于提供高阶数据汇总与中间分析步骤的记录。
如需额外说明,请联系论文作者。相关集合材料可访问:https://doi.org/10.25452/figshare.plus.c.7052513
提供机构:
Figshare+
创建时间:
2024-02-01



