Pond water SIP fraction microbial communities from Walnut Creek, CA, United States - JGIPond_D_40pct_30ng_1_1.7518 metagenome. pond metagenome

SIP metagenome fraction of pond sample from Joint Genome Institute, Walnut Creek spiked with Pseudomonas putida & Escherichia coli bacterial isolates (for each isolate 30ng of DNA labeled at 40pct with C13 were added); replicate number 1. This fraction has a SIP fraction number 5 and DNA density of 1.7518 g/uL. This is part of a larger study to identify the limit of detection of JGI SIP metagenomics pipeline.

本样本为核桃溪联合基因组研究所（Joint Genome Institute, JGI）获取的池塘样本的宏基因组组分（metagenome fraction），经稳定同位素探针（Stable Isotope Probing, SIP）处理，掺入了恶臭假单胞菌（Pseudomonas putida）与大肠杆菌（Escherichia coli）的细菌分离株——每株分离菌均添加30ng被13C标记比例为40%的DNA；该样本为第1次重复样本。此组分的SIP编号为5，DNA密度为1.7518 g/uL。本样本属于一项旨在明确联合基因组研究所SIP宏基因组分析流程检测限的更大规模研究的组成部分。