Effect of depletion of TRIM25 and inhibition of p300 on gene expression in breast cancer cells. Effect of depletion of TRIM25 and inhibition of p300 on gene expression in breast cancer cells

To analyze the effect of TRIM25 on gene expression, TRIM25 was knocked out in MCF7 cells. To understand a possible contribution of p300 on this gene regulation, p300 was inhibited. RNA seq was performed to see changes in the whole transcriptome Overall design: TRIM25 was targeted by CRISPR/Cas9 or p300 was inhibited by treatment with CCS1477 for 20 h in MCF7 cells. Total RNA was prepared and sequenced

为分析TRIM25对基因表达的影响，研究人员在MCF7细胞中敲除了TRIM25。为探究p300在该基因调控过程中的潜在作用，研究人员对p300进行了抑制处理。本研究通过RNA测序（RNA-seq）检测全转录组的表达变化。
实验整体设计：在MCF7细胞中，分别通过CRISPR/Cas9靶向敲除TRIM25，或使用CCS1477处理20小时以抑制p300活性。随后提取总RNA并进行测序。