Soybean root phenotyping dataset and weather dataset from PPAC (Piney Purdue Agricultural Center), IN
收藏资源简介:
This data repository contains records of root phenotypes which were collected in the Pinney Purdue Agricultural Center (PPAC) (Wanatah, Indiana, USA). Briefly, plants were grown in the two sites within PPAC that differed in soil texture, sandy loam and clay loam. In each site, there were three replications per genotype. Plants were hand-sown in single-row plots 3 m in length (2.1 m short row with 0.9 m alley) to a depth of 2.5 cm to 3.8 cm. Roots were collected for all genotypes and both sites across three different developmental timepoints, giving rise to 432 samples altogether. Plant samples were randomly selected from each single row plot for root sample collection. One sample per plot was collected at a time to ensure accuracy. We used shovels to excavate samples, taking care to minimize damage to the root systems, and samples were stored in ziploc bags for transportation to the laboratory. Images of roots were collected using an imaging platform made up of a DSLR camera, copy stand, and light box. Trait extraction was carried out in WinRhizo and ImageJ. The following files contain the data with the root phenotypes and weather data for that season.
本数据集仓库收录了美国印第安纳州瓦纳塔市皮尼普渡农业中心(Pinney Purdue Agricultural Center, PPAC)的根系表型记录。简要而言,本研究在PPAC内两个土壤质地各异的试验点开展,分别为砂壤土与黏壤土。每个试验点中,每份供试基因型设置3次生物学重复。作物以单行小区形式人工播种,小区行长3米(含0.9米操作过道的2.1米种植行),播种深度为2.5厘米至3.8厘米。 在三个不同的生育时期,针对所有供试基因型与两个试验点的作物开展根系采样,总计获得432份样本。从每个单行小区中随机选取植株用于根系采集,每次仅采集1份单株样本以保障数据准确性。研究人员使用铁锹挖掘样本,尽量降低对根系系统的损伤,采集后的样本封装于Ziploc品牌自封保鲜袋中,转运至实验室储存。 根系图像通过由数码单反相机(DSLR)、翻拍架与光源箱组成的成像平台完成采集。表型性状提取工作依托WinRhizo与ImageJ软件开展。 以下文件包含本试验季的根系表型数据与同期气象数据。



