Bacillus cereus Raw sequence reads. Bacillus cereus

RNAs can begin to fold immediately after emerging from RNA polymerase during transcription. Interactions between nascent RNAs and ligands during cotranscriptional folding can direct the formation of alternative RNA structures, a feature exploited by non-coding RNAs called riboswitches to make gene regulatory decisions. These sequencing datasets were generated using cotranscriptional SHAPE-Seq which measures structural information of nascent RNAs during transcription. The datasets describe the in vitro folding pathways of the B. cereus Fluoride riboswitch using a biotin-streptavidin RNA polymerase roadblocking strategy.

核糖核酸（RNA）可在转录过程中从RNA聚合酶（RNA polymerase）脱离后即刻开始折叠。共转录折叠过程中，新生RNA（nascent RNA）与配体（ligand）的相互作用可引导替代性RNA结构的形成，这一特征被一类名为核糖开关（riboswitch）的非编码RNA（non-coding RNA）所利用，以做出基因调控决策。本测序数据集通过共转录SHAPE-Seq技术生成，该技术可在转录过程中检测新生RNA的结构信息。该数据集借助生物素-链霉亲和素（biotin-streptavidin）RNA聚合酶阻断策略，描述了蜡样芽孢杆菌（B. cereus）氟化物核糖开关的体外折叠通路。