Microarray analysis of gene expression induced by Phytophthora infestans in tomato leaves

Phytophthora infestans is most notorious oomycete causing a devastating disease on tomato called late blight. The molecular mechanisms involved in host-parasite interaction is still unexplored well. Investigation of changes in gene expression profile after pathogen infection to find out the mechanisms involved in infection process Second full expanded leaves from both healthy tomato plants (non-inoculated) and diseased tomato plants inoculated with Phytophthora infestans inoculum were used to extract total RNA for microarry analysis 12 hours post inoculation time.

致病疫霉（Phytophthora infestans）是最为臭名昭著的卵菌之一，可引发番茄的毁灭性病害——晚疫病。目前，其参与寄主-寄生菌互作的分子机制尚未得到充分阐释。为探究病原菌侵染过程的相关分子机制，本研究拟分析病原菌侵染后宿主的基因表达谱变化。实验选取健康（未接种）番茄植株与接种致病疫霉接种液的番茄植株，在接种后12小时采集二者的第二片完全展开叶片，提取总RNA用于微阵列（microarray）分析。