<b>Complement inhibition reshapes tumor microenvironment and enhances clinical efficacy in immunotherapy</b>

We conducted single-cell transcriptome (scRNA-seq) and whole-exome sequencing (WES) analyses on 9 esophageal cancer patients, consisting of three cases with complement mutations and six cases without mutations, resulting in a total of 52,727 cells after quality control. The findings indicate a significant increase in the proportion of NK cells and T cells among CD45+ cells in the complement mutation group, whereas the proportion of monocytes and macrophages decreases. Furthermore, gene set enrichment analysis (GSEA) of malignant cells reveals significant enrichment of pathways such as "interferon alpha response," "KRAS signaling DN," and "interferon gamma response" in the complement mutation group.本研究对9例食管癌患者开展了单细胞转录组测序（single-cell RNA sequencing，scRNA-seq）及全外显子组测序（whole-exome sequencing，WES）分析；纳入患者包括3例携带补体突变者与6例无突变者，经质量控制后共获得52727个细胞。研究结果显示，补体突变组CD45+细胞中自然杀伤细胞（NK cells）与T细胞的占比显著升高，而单核细胞与巨噬细胞的占比则呈下降趋势。此外，针对恶性细胞的基因集富集分析（gene set enrichment analysis，GSEA）结果表明，补体突变组显著富集了"干扰素α应答"、"KRAS信号通路下调"及"干扰素γ应答"等通路。