Characterization of distinct classes of differential gene expression in osteoblast cultures from non-syndromic craniosynostosis bone. Homo sapiens

We report on explant osteoblast cultures from human patients, demonstrating that there are at least three sub-types of non-syndromic craniosynostosis defined by similarity of gene expression profiles. Overall design: Osteoblast growth in culture, 23 craniosynostosis skull samples (7 metopic; 8 coronal; 3 lambdoid; 5 sagittal) and 8 normal (4 cranial bones and 4 long bones)

本研究报道了源自人类患者的外植体成骨细胞培养体系，证实至少存在三类以基因表达谱相似性为判定依据的非综合征性颅缝早闭亚型。整体实验设计：成骨细胞体外培养实验共纳入23例颅缝早闭患者颅骨样本，其中额缝型7例、冠状缝型8例、人字缝型3例、矢状缝型5例；另纳入8例正常对照样本，含4例颅骨骨组织与4例长骨组织。