Targeted epigenetic modulation using a DNA-based histone deacetylase inhibitor enhances cardiomyogenesis in mouse embryonic stem cells (ChIP-Seq)

To determine how SAHA-PIP G can trigger the expression of the mesendoderm-related genes in mouse ES cells, chromatin immunoprecipitation (ChIP-seq) analysis was carried out with an antibody against the histone H3 lysine 4 trimethylation (H3K4me3) using EBs harvested on day 0 Overall design: Examination of H3K4me3 in 2 embryonic body types that with/without 1 µM SAHA-PIP G treatment.

为明确SAHA-PIP G诱导小鼠胚胎干细胞（mouse ES cells）中内胚层相关基因的表达机制，本研究使用针对组蛋白H3赖氨酸4三甲基化（H3K4me3）的抗体，对第0天收获的胚状体（embryoid bodies, EBs）开展染色质免疫共沉淀测序（ChIP-seq）分析。实验整体设计：检测两种胚状体样本的H3K4me3修饰水平，分别为经1 μM SAHA-PIP G处理组与未处理组。