Physiological transcriptional profiling of murine osteocytes from calvaria and long bones. Physiological transcriptional profiling of murine osteocytes from calvaria and long bones

Different bones of the skeleton originate from three distinct embryonic lineages. Osteoblasts derived from bones of different embryonic origin displayed cell intrinsic difference. Osteocytes are terminally differentiated osteoblasts which displays a unique genetic makeup and a distinct morphology. It is not know if osteocytes display a cell intrinsic differences depeding on their origin. To understand the difference in the gene expression profiles of osteocytes of three different embryonic origins we examined osteocytes from three different bones neural crest origin-frontal bone, paraxial mesoderm origin-parietal bone, and lateral plate mesoderm- femur bone. Overall design: Examine the changes in the transcriptome in frontal, parietal and femur osteocytes.

骨骼系统的不同骨组织起源于三种截然不同的胚胎谱系。源自不同胚胎起源骨组织的成骨细胞(Osteoblasts)存在细胞内在差异。骨细胞(Osteocytes)是终末分化的成骨细胞，具有独特的基因组成与特异的形态学特征。目前尚不明确骨细胞是否会因其起源不同而表现出细胞内在差异。为探究三种不同胚胎起源骨细胞的基因表达谱差异，我们对三类不同骨组织中的骨细胞进行了检测：分别为神经嵴起源的额骨、轴旁中胚层起源的顶骨，以及侧板中胚层起源的股骨。实验整体设计：检测额骨、顶骨及股骨骨细胞的转录组(transcriptome)表达变化。