Genome-wide identification of differentially methylated promoters and enhancers associated with response to anti-PD-1 therapy in non-small cell lung cancer

The purpose of this study is to compare transcriptional profiles of non-small-cell lung cancer between responder patients and non-responder patients during anti-PD-1 treatment. Overall design: 16 patients sample were obtained as 11 fresh samples and 5 formalin-fixed paraffin-embedded (FFPE) samples. Each sample was subsequently prepared by TruSeq RNA Access Library Prep Guide Part # 15049525 Rev. B with TruSeq RNA Access Library Prep Kit. RNA-sequencing was performed by HiSeq 2500 with HiSeq 2500 System User Guide Document # 15035786 v01 HCS 2.2.70.

本研究旨在对比抗PD-1治疗期间，应答患者与非应答患者的非小细胞肺癌转录组谱。总体实验设计：共纳入16例患者样本，其中11例为新鲜样本，5例为福尔马林固定石蜡包埋（formalin-fixed paraffin-embedded, FFPE）样本。所有样本均按照《TruSeq RNA Access文库制备指南 第15049525号 修订版B》，使用TruSeq RNA Access文库制备试剂盒完成文库制备。随后采用HiSeq 2500测序系统，参照《HiSeq 2500系统用户指南文档 第15035786号 v01 HCS 2.2.70》完成RNA测序。