Single-cell transcriptome sequencing

Raw reads from single-cell RNA-sequencing of peripheral blood of five TET2 mutation carriers as well as three non-carrier family members. Single-cells were captured into 10x barcoded gel beads and RNA-sequencing library preparation was done using Chromium Single Cell 3' v2 chemistry (10x Genomics, Pleasanton, CA, USA). Sequencing was performed as recommended with 98bp length of read 2 using HiSeq4000 sequencer.

本数据集包含五位TET2突变携带者以及三位非携带者家庭成员的外周血单个细胞的RNA测序原始读数。单个细胞被捕获到10x条形码凝胶珠中，并采用Chromium Single Cell 3’ v2化学法（10x Genomics，Pleasanton，加利福尼亚州，美国）进行RNA测序文库的制备。测序过程遵循推荐方案，使用HiSeq4000测序仪进行，第二读长为98碱基对。