Whole genome sequencing of Vibriophage escapes from a satellite packaging inhibitor

Total DNA was collected from infected cultures and used to generate DNA libraries. DNA libraries were prepared at the Microbial Genome Sequencing Center (Pittsburgh, PA) using the Illumina DNA Prep kit and IDT 10bp UDI indices, and paired-end sequencing (2x150 bp) was performed on an Illumina NextSeq2000 (Pittsburg, PA)

从感染培养物中提取总DNA（Total DNA），并将其用于构建DNA文库（DNA libraries）。DNA文库的制备工作于微生物基因组测序中心（Microbial Genome Sequencing Center，美国宾夕法尼亚州匹兹堡市）完成，采用Illumina DNA制备试剂盒（Illumina DNA Prep kit）与IDT 10bp UDI索引（IDT 10bp UDI indices），并依托Illumina NextSeq2000测序平台（美国宾夕法尼亚州匹兹堡市）开展双端测序（paired-end sequencing），测序读长设置为2×150碱基对（2x150 bp）。