E2F6-mediated ceRNA and epigenetic silencing of miR-193a promote cancer stemness and immunoevasion in ovarian cancer. E2F6-mediated ceRNA and epigenetic silencing of miR-193a promote cancer stemness and immunoevasion in ovarian cancer

To investigate the knockdown effect of EZH2 and E2F6, RNA-sequencing (RNA-seq) was performed to analyze the genome-wide changes by the knockdown of EZH2 or E2F6 in CP70 ovarian cancer cells. Overall design: CP70 ovarian cancer cells were infected with pLKO TRC005 (vector control) or pLKO TRC005 shE2F6 (treatment) or pLKO.1-puro shEZH2(treatment).

为探究EZH2与E2F6的基因敲低效应，本研究通过RNA测序（RNA-sequencing, RNA-seq）分析CP70卵巢癌细胞中敲低EZH2或E2F6后全基因组水平的表达变化。总体实验设计：将CP70卵巢癌细胞分别感染pLKO TRC005（空载体对照）、pLKO TRC005 shE2F6（处理组）以及pLKO.1-puro shEZH2（处理组）。