author checklist for animal research by arriveguideline.pdf

This data is the extend data of research titled <strong>Gingival Healing Response in Periodontitis Case of Wistar Rat Model After Application of Sarang Semut Plant (</strong><em><strong>Myrmecodia pendens) </strong></em><strong>Extract</strong> by fill in the Arrive guideline set 10 and recommend set. <br> <strong>Background: </strong>Infected periodontal pocket by microorganisms plays an important role in the occurrence of periodontal tissue necrosis and the formation of periapical abnormalities. Therefore, periodontal pocket treatment should be carried out with the aim of eliminating or reducing the microbial population and biomechanically removing necrotic tissue which is a medium for microbial growth and preventing re-infection. One of the medicinal plants from the Kalimantan forest that has great potential is Sarang Semut (<em>Myrmecodia pendens</em>). This study aims to determine the response of gingival healing in the case of periodontitis in the wistar rat model after the application of Sarang Semut plant (<em>Myrmecodia pendens</em>) extract. <strong>Material and Method:</strong> Sarang Semut plant (<em>Myrmecodia pendens</em>) extract dilution to concentration of 2.5%, 5% and 10% was carried out at the Microbiology Laboratory followed by research using experimental animals until gingival excision. Preparation of excised gingival and reading tissue preparations was carried out at the Veterinary Medicine Laboratory. This research is a laboratory experimental research and used post-test with control group design. <strong>Results:</strong> The results showed that ethanol extract of Sarang Semut can increase the healing response of the gingiva that has been induced by periodontitis bacteria. There is three concentration that have been given 2.5%, 5%, and 10%. The smallest concentration provide healing area. <strong>Conclusion:</strong> Gingival healing response in periodontitis cases of wistar rat model after the application of Sarang Semut plant (<em>Myrmecodia pendens</em>) extract showed results that resembled positive control on the application of 2.5% with clinical signs on the rat’s gingiva that experienced changes in color, contour, and blending.

本数据集为题为<strong>《Wistar大鼠（Wistar Rat）模型牙周炎病例应用Sarang Semut（Myrmecodia pendens）提取物后的牙龈愈合反应》</strong>的研究补充数据，本研究遵循ARRIVE指南第10项及相关推荐方案完成。<br><strong>背景: </strong>微生物感染牙周袋在牙周组织坏死及根尖周异常的发生中发挥关键作用。因此，牙周袋治疗的目标应为消除或减少微生物种群，机械清除作为微生物生长介质的坏死组织，并预防再次感染。加里曼丹森林中极具开发潜力的药用植物之一为Sarang Semut（Myrmecodia pendens）。本研究旨在探究应用Sarang Semut提取物后，Wistar大鼠模型牙周炎病例的牙龈愈合反应。<br><strong>材料与方法:</strong> 将Sarang Semut提取物稀释至2.5%、5%及10%的浓度，实验操作于微生物学实验室完成；随后使用实验动物开展研究，直至牙龈组织摘除。牙龈摘除组织的制备及组织切片阅片工作在兽医学实验室完成。本研究为实验室实验研究，采用单后测对照组设计。<br><strong>结果:</strong> 结果显示，Sarang Semut乙醇提取物可增强经牙周炎细菌诱导的牙龈愈合反应。本次实验设置了2.5%、5%及10%三个浓度梯度，其中最低浓度组的愈合面积最小。<br><strong>结论:</strong> 应用Sarang Semut提取物后，Wistar大鼠模型牙周炎病例的牙龈愈合反应结果显示，2.5%浓度组的效果与阳性对照组相近，该组大鼠牙龈的临床体征出现颜色、轮廓及组织贴合度的改善。