ExtFig3D_qPCR_BCAP31_R1_LG274.xlsx

<em>HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the BCAP1, INSIG1, SLC7A5, STOML2, YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X. RNA was extracted and luciferase RNA and 18S rRNA levels were quantified by qRT-PCR</em>

将HEK293T ishXrn1细胞经多西环素处理3~4天以诱导Xrn1基因敲低，随后转染携带BCAP1、INSIG1、SLC7A5、STOML2、YKT6及TUBA1B基因来源的99 bp插入片段的荧光素酶报告质粒；在实验标注的实验组中，同步共转染PR8 PA-X。提取细胞总RNA后，通过qRT-PCR对荧光素酶RNA与18S rRNA的水平进行定量检测。