Data for: Assessment of genetic diversity and protein content of Scandinavian peas (Pisum sativum)
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https://datadryad.org/dataset/doi:10.5061/dryad.d7wm37q6b
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资源简介:
We produced homogeneous lines of 227 pea accessions from the Nordic
Genetic Resource Center by single seed descent. The genetic diversity
among these, mostly Scandinavian accessions, was investigated using three
microsatellite markers, A9, AC58 and AA5. The microsatellites were highly
informative and separated 153 of 194 accessions on a Neighbor Joining
topology. The high polymorphism information content (PIC) values between
0.87 and 0.91 indicated that the gene bank material contains a large
number of pea accessions with different breeding. The peas were grown in
the field for two years and seed protein content showed variation between
9.3% and 34.1% over years and accessions. Mean thousand seed weight was
152.05 g. More than 10 accessions had protein content above 28% showing
that the collection has potential as a breeding nursery for high-protein
pea.
本研究通过单粒传法从北欧遗传资源中心(Nordic Genetic Resource Center)构建得到227份豌豆种质的纯合株系。供试种质大多源自斯堪的纳维亚地区,研究人员利用A9、AC58和AA5这3个微卫星标记对其遗传多样性展开分析。结果显示,所选微卫星标记具有极高的信息价值,在邻接(Neighbor Joining)拓扑结构中成功区分了194份种质中的153份。其多态性信息含量(polymorphism information content, PIC)值介于0.87至0.91之间,表明该基因库馆藏材料包含大量遗传背景各异的豌豆种质。
本研究将供试豌豆于田间连续种植两年,结果显示不同年份及不同种质间的种子蛋白质含量介于9.3%至34.1%,存在广泛变异。供试材料的平均千粒重为152.05 g。其中超过10份种质的蛋白质含量超过28%,表明该种质集合具备作为高蛋白豌豆育种选育圃的潜力。
提供机构:
Dryad
创建时间:
2023-09-08



