Unraveling the Role of Srsf1 in Brown Adipose Tissue Thermogenic Function through Single-Nucleus RNA Sequencing.

Single-nucleus RNA sequencing reveals that Srsf1 absence alters the cell type composition and differentiation trajectories of BAT. Overall design: Interscapular brown adipose tissue was harvested from 8-week-old control and adipose tissue-specific Srsf1-knockout mice for experiment. In order to obtain single-cell suspension, fresh tissue samples were cut and lysed in nucleus lysis solution. The nuclei obtained were subsequently used for sequencing.

单细胞核RNA测序（single-nucleus RNA sequencing）结果显示，Srsf1缺失会改变棕色脂肪组织（brown adipose tissue, BAT）的细胞类型组成与分化轨迹。实验设计：从8周龄对照组及脂肪组织特异性Srsf1敲除小鼠身上取材肩胛间棕色脂肪组织用于实验。为制备单细胞悬液，将新鲜组织样本切割后置于细胞核裂解液中进行裂解，所获得的细胞核随后用于测序实验。