Bulk RNA-seq of proliferating cells in the mouse stomach corpus epithelium from FUCCI mouse.

The purpose of this bulk RNA-seq experiment is to identify candidate genes that can mark proliferating isthmus progenitors in the mouse stomach corpus epithelium. The cycling isthmus progenitors were isolated based on mVenus expression in FUCCI mouse and used for bulk RNA-seq data generation. The corpus epithelium was stained with E-cadherin and E-cadherin+ cells were also sorted and used for the control.

本批量RNA测序（bulk RNA-seq）实验旨在筛选可作为小鼠胃体上皮中增殖性峡部祖细胞标记物的候选基因。研究人员基于FUCCI小鼠体内的mVenus表达特征，分离得到循环增殖的峡部祖细胞，用于生成批量RNA测序数据。同时，将胃体上皮经E-钙粘蛋白（E-cadherin）染色后分选得到的E-钙粘蛋白阳性细胞，用作本次实验的对照样本。