Profiling of human monoclonal antibodies targeting SARS-CoV-2 Omicron variant. Profiling of human monoclonal antibodies targeting SARS-CoV-2 Omicron variant

We generated LNP-mRNA encoding B.1.1.529 SARS-CoV-2 spike, and intramuscularly administered it in a human IgG and IgK knock-in mouse. Single cell VDJ-seq unveiled the sequences of human monoclonal antibodies targeting the B.1.1.529 SARS-CoV-2 spike protein. Overall design: Three sets of B cells were isolated and processed from the spleen, lymph nodes, bone marrows, or peripheral blood of the immunized mouse and then analyzed by scVDJ-seq.

我们制备了编码B.1.1.529型新型冠状病毒（SARS-CoV-2）刺突蛋白的脂质纳米颗粒-mRNA（LNP-mRNA），并将其肌内免疫接种至人IgG和IgK敲入小鼠体内。通过单细胞VDJ测序（Single cell VDJ-seq），我们解析了靶向B.1.1.529型SARS-CoV-2刺突蛋白的人源单克隆抗体序列。
总体实验设计：从免疫小鼠的脾脏、淋巴结、骨髓或外周血中分离并处理三组B细胞，随后通过scVDJ-seq完成测序分析。