Gene expression data from 1833 cells expressing RKIP vs control

RKIP regulates human breast tumor metastasis. We use gene expression array analysis to identify genes regulated by RKIP in human breast cancer cells. Total RNAs were extracted from 1833 cells expressing mutant RKIP (S153E-RKIP, a more potent Raf-1 inhibitor) and vector control. Affymetrix GeneChip hgu133plus2.0 Arrays were performed to detail the gene expression and identify the genes regulated by RKIP in human breast cancer cells.

RKIP可调控人类乳腺肿瘤转移。本研究采用基因表达芯片分析技术，筛选人类乳腺癌细胞中受RKIP调控的基因。实验从表达突变型RKIP（S153E-RKIP，一种更高效的Raf-1抑制剂）的1833细胞与空载体对照细胞中提取总RNA，并通过Affymetrix GeneChip hgu133plus2.0芯片完成检测，以全面解析基因表达谱并鉴定人类乳腺癌细胞中受RKIP调控的基因。